Nahuel Guzman saved two penalties but Tigres' title defense came to an end as Monterrey earned a 4-3 aggregate victory in the Clasico Regio quarterfinals.
Antonio "Turco" Mohamed's side took a comprehensive 3-1 lead in the first leg, but it took just 11 minutes for the visitors to jump in front when Jesus Dueñas headed home from a Javier Aquino cross.
Tigres then chased their way towards a second when a Israel Jimenez through ball found Andre-Pierre Gignac, who laid the ball off for Rafael Sobis to tap into an empty net.
Early in the second half, though, a penalty was awarded for a push on Rogelio Funes-Mori. Dorlan Pabon stepped up, only for Tigres goalkeeper Nahuel Guzman make a great save to keep his side's semifinal hopes alive.
In the 69th minute, Juninho was sent off for a second bookable offense, leaving Tigres down to ten men and still needing a goal to overturn the tie.
Juninho would rue his decision, and from the following set-piece, Cesar Montes found himself unmarked to fire home the 4-3 and give Monterrey the lead.
Funes-Mori could and perhaps should have sealed it in the 85th minute. A second penalty was given after a handball was wrongly given inside the area, but Nahuel Guzman once again stood tall to save his second penalty of the night.
Back at the other end, Tigres failed to produce an attacking response as Monterrey sealed their passage to the Liga MX semifinals.
Meanwhile, Leon will join Monterrey after La Fiera earned a comfortable 4-1 win over Monarcas Morelia.
With the score at 1-1 aggregate after the first leg, Luis Montes put Leon in front with his second goal of the series on 34 minutes, tapping in from Diego Novaretti's headed pass.
Leon appeared in control of the game, and made it two only seconds into the second half when Montes and Mauro Boselli passed their way into the box straight from kickoff to put things at 3-1.
Elias Hernandez then added a third, finishing a quick breakaway by firing his shot past Carlos Felipe Rodriguez.
Scorer turned provider, and Hernandez sent a through ball for Montes, who scored his second of the night to increase la Fiera's lead.
In the 89th minute, Rodrigo Millar scored a superb volley, but it was too little too late as Leon reached the final four of the competition.